キャンパスライフ

キャンパスライフ 在学生・教職員の活躍

平成28年度大阪薬科大学研究振興基金助成の受賞者が決定

 大阪薬科大学研究振興基金助成は、若手専任教員が本学で行った研究成果の中で、特に優れた研究論文を発表した者を顕彰し、更なる研究活動の発展を支援することを目的としたものです。
 平成28年度は下記の3名が受賞となりました。

助成対象者 門田 和紀 講師(製剤設計学研究室)
論文名 Hybridization of polyvinylpyrrolidone to a binary composite of curcumin/α-glucosyl stevia improves both oral absorption and photochemical stability of curcumin.
掲載雑誌 Food Chemistry.213,668-674(2016)
概要

本研究では,難水溶性の機能性食品成分として知られているクルクミンに対して機能性食品添加剤である酵素処理したステビア(糖転移ステビア)を製剤担体として使用し,その溶解性,光安定性及び経口吸収性の改善を試みた.クルクミンと糖転移ステビアの 2 成分系において,過飽和状態の維持は困難であったが,3 成分目として Polyvinylpyrrolidone (PVP) K-30 を添加し,相乗的な溶解度改善また過飽和安定化に成功した.製剤化による クルクミンの溶解性改善を評価するため,溶出液として水を用いた溶出試験を実施した結果,結晶 クルクミンの溶解度に対して約 8 倍の溶解度を示した.更に,熱湿度条件下で保存後の製剤においてもその溶出挙動はほとんど変化しなかった.

また,結晶クルクミン (100 mg/kg) 及び本製剤 (20 mg-CUR/kg) をラットに対して経口投与後の血中濃度プロファイルから経口吸収性を評価した.結晶クルクミンの投与群において,血漿中 CUR 濃度の上昇は非常に限られたものであったのに対し,製剤投与群においては Cmax 及び AUC0-180min の値はそれぞれ 上昇しており,この値から算出した相対的バイオアベイラビリティは約 7 倍向上していた.以上の結果から,クルクミン含有機能性粒子開発において,糖転移ステビアおよびPVP K30は効果的な役割をすることを明らかとした.
助成対象者 東 剛志 助教(薬品分析化学研究室)
論文名 Detection of pharmaceuticals and phytochemicals together with their metabolites in hospital effluents in Japan, and their contribution to sewage treatment plant influents
掲載雑誌 Science of the Total Environment. 548–549, 189–197 (2016)
概要

The occurrence of 41 pharmaceuticals and phytochemicals (PPs) including their metabolites was surveyed in hospital effluent in an urban area of Japan. A detailed survey of sewage treatment plant (STP) influent and effluent, and river water was also conducted. Finally, mass balances with mass fluxes of the target PPs through the water flow were evaluated and the degree of contribution of hospital effluent to the environmental discharge was estimated.
The results indicate that 38 compounds were detectable in hospital effluent over a wide concentration range from ng/L to μg/L, with a maximum of 92 μg/L. The contributions of PPs in the hospital effluent to STP influent varied widely from <0.1% to 14.8%. Although almost all of the remaining components could be removed below
1.0 ng/L at STPs by the addition of ozone treatment, a number of PPs still remained above 10 ng/L in STP effluent.

These findings suggest the importance of applying highly developed treatments to hospital effluents and at STPs in the future to reduce the environmental risks posed by PPs. To our knowledge, this is the first demonstration of the presence of two conjugated metabolites of acetaminophen, acetaminophen glucuronide and acetaminophen sulfate, as well as of loxoprofen and loxoprofen alcohol, in hospital effluent, STP, and river waters.

助成対象者 小池 敦資 助手(生体防御学研究室)
論文名 Simultaneous addition of shikonin and its derivatives with lipopolysaccharide induces rapid macrophage death
掲載雑誌 Biological and Pharmaceutical Bulletin. 39, 969-976 (2016)
概要

Macrophages play pivotal roles in inflammatory responses. Previous studies showed that various natural products exert antiinflammatory effects by regulating macrophage activation. Recent studies have shown that shikonin (SHK) and its derivatives (β-hydroxyisovalerylshikonin, acetylshikonin, and isobutylshikonin), which are 1,4-naphthoquinone pigments extracted from the roots of Lithospermum erythrorhizon, have various pharmacological, including antiinflammatory and antitumor, effects. Even though there have been many studies on the antiinflammatory activities of SHK derivatives, only a few have described their direct effects on macrophages. We investigated the effects of SHK derivatives on lipopolysaccharide (LPS)-treated macrophages. Low doses of SHK derivatives induced significant macrophage cytotoxicity (mouse macrophage-like J774.1/JA-4 cells and mouse peritoneal macrophages) in the presence of LPS. SHK activated caspases-3 and -7, which led to DNA fragmentation, but this cytotoxicity was prevented through a pan-caspase inhibitor in LPS-treated JA-4 cells. Maximal cytotoxic effects were achieved when SHK was added immediately before LPS addition. These results indicate that SHK derivatives induce caspase-dependent apoptotic cell death of LPS-treated macrophages and suggest that SHK acts during an early stage of LPS signaling.


ページトップへ